Immunofluorescent analysis of RAD51 in nuclear foci of U2OS cells using a RAD51 monoclonal antibody (Product # MA1-23271) at a dilution of 1:1000. Cells were pre-extracted with CSK buffer and fixed with 4% PFA. DAPI was used to counterstain the nucleus. Scale bar, 10 µm.
|Tested species reactivity||Chicken, Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Full length Rad51 protein (amino acids 1-338) expressed in E. coli.|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA1-23271 detects Rad51 in human and chicken samples.
MA1-23271 has been successfully used in immunofluorescence, immunoprecipitation, IHC-P, and Western blot procedures. A suggested positive control for this product is T24.
The MA1-23271 immunogen is full length Rad51 protein (amino acids 1-338) expressed in E. coli.
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Rad51, a 37 kDa protein, is the human homologue of E. coli RecA protein and a member of the RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported that BRCA1 interacts with Rad51 and disease-causing mutations have been found in the BRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction is important for tumor suppression. BRCA2 and Rad51 have also been shown to interact by direct binding of the BRC repeats located within exon 11 of BRCA2. When these repeats are deleted, the interaction is lost and cells become hypersensitive to the DNA damage caused by methyl methanesulfonate (MMS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage.
MA1-23271 was used in immunocytochemistry to study the response to double-stranded breaks at the nucleolar organizer regions.
|van Sluis M,McStay B||Genes and development (29:1151)||2015|