Immunofluorescent analysis of RAD51 (green) showing staining in the cytoplasm and nucleus of H1299 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a RAD51 monoclonal antibody (Product # MA5-14419) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Yeast, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant Rad51 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:50-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14419 targets RAD51 in IHC (P), IP, ICC/IF and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-14419 immunogen is recombinant Rad51 protein.
Rad51 (a eukaryotic homologue of Escherichia coli RecA) is similar to RecA, both biochemically and structurally: It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57 and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD 51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The DNA damage/repair cascade in glioblastoma cell lines after chemotherapeutic agent treatment.
MA5-14419 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to analyze chemotherapeutic agent treatment and the DNA damage/repair cascade in glioblastoma cell lines
|Annovazzi L,Caldera V,Mellai M,Riganti C,Battaglia L,Chirio D,Melcarne A,Schiffer D||International journal of oncology (46:2299)||2015|
A single cohesin complex performs mitotic and meiotic functions in the protist tetrahymena.
MA5-14419 was used in immunocytochemistry to study the ability of a single Tetrahymena cohesin complex to perform both mitotic and meiotic functions
|Howard-Till RA,Lukaszewicz A,Novatchkova M,Loidl J||PLoS genetics (9:null)||2013|
The Tetrahymena meiotic chromosome bouquet is organized by centromeres and promotes interhomolog recombination.
MA5-14419 was used in immunocytochemistry to study the essential role of centromere function in formation of the chromosome bouquet and inter-homolog recombination in Tetrahymena
|Loidl J,Lukaszewicz A,Howard-Till RA,Koestler T||Journal of cell science (125:5873)||2012|
Nucleostemin prevents telomere damage by promoting PML-IV recruitment to SUMOylated TRF1.
MA5-14419 was used in immunocytochemistry to study the roles of PML-IV and SUMOylated TRF1 in the mechanism by which nucleostemin protects telomeres against damage
|Hsu JK,Lin T,Tsai RY||The Journal of cell biology (197:613)||2012|
The role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells.
MA5-14419 was used in immunocytochemistry to study the role of meiotic cohesin REC8 in chromosome segregation in gamma irradiation-induced endopolyploid tumour cells
|Erenpreisa J,Cragg MS,Salmina K,Hausmann M,Scherthan H||Experimental cell research (315:2593)||2009|
DNA double-strand breaks, but not crossovers, are required for the reorganization of meiotic nuclei in Tetrahymena.
MA5-14419 was used in immunocytochemistry to study the role of DNA double-strand breaks in the reorganization of meiotic nuclei in Tetrahymena
|Mochizuki K,Novatchkova M,Loidl J||Journal of cell science (121:2148)||2008|
Meiotic recombination proteins localize to linear elements in Schizosaccharomyces pombe.
MA5-14419 was used in immunocytochemistry to study the localization of meiotic recombination proteins in Schizosaccharomyces pombe
|Lorenz A,Estreicher A,Kohli J,Loidl J||Chromosoma (115:330)||2006|
Endopolyploid cells produced after severe genotoxic damage have the potential to repair DNA double strand breaks.
MA5-14419 was used in immunocytochemistry to study the role of endopolyploid cells in the repair of DNA double strand breaks
|Ivanov A,Cragg MS,Erenpreisa J,Emzinsh D,Lukman H,Illidge TM||Journal of cell science (116:4095)||2003|
|Not Applicable||Not Cited||
Chiasmatic and achiasmatic inverted meiosis of plants with holocentric chromosomes.
MA5-14419 was used in immunohistochemistry to analyze plants with holocentric chromosomes that undergo chiasmatic and achiasmatic meiosis
|Cabral G,Marques A,Schubert V,Pedrosa-Harand A,Schlögelhofer P||Nature communications (5:null)||2014|
Rad51 expression is a useful predictive factor for the efficacy of neoadjuvant chemoradiotherapy in squamous cell carcinoma of the esophagus.
MA5-14419 was used in immunohistochemistry to study the value of the immunohistochemical expression of Rad51 in predicting the response of patients with squamous cell carcinoma of the esophagus to neoadjuvant chemotherapy
|Nakanoko T,Saeki H,Morita M,Nakashima Y,Ando K,Oki E,Ohga T,Kakeji Y,Toh Y,Maehara Y||Annals of surgical oncology (21:597)||2014|
Human papillomavirus in oral atrophic lichen planus lesions.
MA5-14419 was used in immunohistochemistry to study the involvement of human papillomavirus in oral atrophic lichen planus lesions
|Mattila R,Rautava J,Syrjänen S||Oral oncology (48:980)||2012|
Organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila.
MA5-14419 was used in immunohistochemistry to study the organization and pairing of meiotic chromosomes in the ciliate Tetrahymena thermophila
|Loidl J,Scherthan H||Journal of cell science (117:5791)||2004|
Selenium protection from DNA damage involves a Ref1/p53/Brca1 protein complex.
MA5-14419 was used in immunoprecipitation to study the role of the Ref1-p53-Brca1 protein complex in seleno-L-methionine-mediated protection against DNA damage
|Fischer JL,Lancia JK,Mathur A,Smith ML||Anticancer research (26:899)||2006|
Interaction of radiation- and bleomycin-induced lesions and influence of glutathione level on the interaction.
MA5-14419 was used in western blot to study the effect of the glutathione levels on the interaction of radiation- and bleomycin-induced lesions
|Dutta A,Chakraborty A,Saha A,Ray S,Chatterjee A||Mutagenesis (20:329)||2005|
Mechanisms of enhanced radiation response following epidermal growth factor receptor signaling inhibition by erlotinib (Tarceva).
MA5-14419 was used in western blot to study the therapeutic efficacy of epidermal growth factor receptor signaling inhibitor erlotinib and its mechanism
|Chinnaiyan P,Huang S,Vallabhaneni G,Armstrong E,Varambally S,Tomlins SA,Chinnaiyan AM,Harari PM||Cancer research (65:3328)||2005|
Analysis of murine Brca2 reveals conservation of protein-protein interactions but differences in nuclear localization signals.
MA5-14419 was used in western blot to compare the protein-protein interactions and nuclear localization signals of human and mouse BRCA2
|Sarkisian CJ,Master SR,Huber LJ,Ha SI,Chodosh LA||The Journal of biological chemistry (276:37640)||2001|