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|Tested species reactivity||Eubacteria|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant RecA expressed in E. coli.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4925 detects RecA in E. coli samples.
PA1-4925 has been successfully used in Western blot, ELISA, and immunoprecipitation procedures. By western blot the antibody detects a ~38 kDa protein (RecA) in E. coli samples.
PA1-4925 immunogen corresponds to recombinant RecA protein.
Store at 4°C short term or at -80°C for long term.
E. coli RecA protein plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its co-protease activity after DNA damage, and induces many proteins related to DNA repair, including RecA itself .
Since RecA has many functions and interacts with various proteins in the cell, the protein complexes can be detected by immunoprecipitaion with the anti-RecA antibody.
RecA forms foci on nucleoids in the cells after exposure to DNA damaging treatments. The foci is visualized by immuno-staining of the cells using this antibody as the first antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.