Invitrogen

RAD51 Monoclonal Antibody (3C10)

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RAD51 Antibody (35-6500) in WB

Western blot analysis of HeLa cell lysates using Ms anti-RAD51 (Product # 35-6500).

Product Image Gallery

RAD51 Antibody — Figure 4 Imatinib increases p53 levels when combined with MX, but not with DXR. Western blot of p53 and RAD 51 in HeLa cells. Imatinib combined with MX increases p53 protein levels in HeLa cells. RAD51 level was slightly reduced in cells treated with imatinib and MX. Cells were treated with MX (0.6 uM), DXR (0.8 uM), imatinib (5 uM) or their combinations for 30 hours. {TM}

Product Details

35-6500

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Human, Pig

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3C10

Immunogen

Purified human RAD51 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533217

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CRISPR/Cas9-Mediated Correction of the FANCD1 Gene in Primary Patient Cells.

International journal of molecular sciences

Skvarova Kramarzova K,Osborn MJ,Webber BR,DeFeo AP,McElroy AN,Kim CJ,Tolar J

35-6500 was used in Western Blotting to show the ability to correct a patient mutation in primary FANCD1 cells in a precise manner.

Wed Jun 14 00:00:00 UTC 2017

High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells.

Molecular & cellular proteomics : MCP

Kanderova V,Kuzilkova D,Stuchly J,Vaskova M,Brdicka T,Fiser K,Hrusak O,Lund-Johansen F,Kalina T

356500 was used to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients

Fri Apr 01 00:00:00 UTC 2016

Inhibition of c-Abl kinase activity renders cancer cells highly sensitive to mitoxantrone.

PloS one

Alpay K,Farshchian M,Tuomela J,Sandholm J,Aittokallio K,Siljamäki E,Kallio M,Kähäri VM,Hietanen S

35-6500 was used in western blot to investigate the sensitization of cancer cells by c-Abl inhibition

Mon May 11 00:00:00 UTC 2015

Exosomes are natural carriers of exogenous siRNA to human cells in vitro.

Cell communication and signaling : CCS

Shtam TA,Kovalev RA,Varfolomeeva EY,Makarov EM,Kil YV,Filatov MV

Mon Nov 18 00:00:00 UTC 2013

Exosomes are natural carriers of exogenous siRNA to human cells in vitro.

Cell communication and signaling : CCS

Shtam TA,Kovalev RA,Varfolomeeva EY,Makarov EM,Kil YV,Filatov MV

Mon Nov 18 00:00:00 UTC 2013

[Human RAD51 recombinase: the role in the cell cycle checkpoint and cellular survival].

Tsitologiia

Shtam TA,Varfolomeeva IIu,Semenova EV,Filatov MV

Fri Mar 13 00:00:00 UTC 2009

[Human RAD51 recombinase: the role in the cell cycle checkpoint and cellular survival].

Tsitologiia

Shtam TA,Varfolomeeva IIu,Semenova EV,Filatov MV

Fri Mar 13 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

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Gene Aliases: BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA

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UniProt ID: (Bovine) Q2KJ94, (Human) Q06609

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Entrez Gene ID: (Bovine) 514749, (Human) 5888, (Pig) 100141309

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Function(s)

recombinase activity chromatin binding double-stranded DNA binding single-stranded DNA binding ATP binding protein C-terminus binding single-stranded DNA-dependent ATP-dependent DNA helicase activity identical protein binding DNA polymerase binding four-way junction DNA binding endodeoxyribonuclease activity protein binding single-stranded DNA-dependent ATPase activity DNA metabolism protein

Process(es)

telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly regulation of protein phosphorylation DNA unwinding involved in DNA replication cellular response to DNA damage stimulus regulation of double-strand break repair via homologous recombination telomere maintenance via telomere lengthening interstrand cross-link repair positive regulation of DNA ligation chromosome organization involved in meiotic cell cycle cellular response to ionizing radiation cellular response to hydroxyurea cellular response to camptothecin replication-born double-strand break repair via sister chromatid exchange mitotic recombination-dependent replication fork processing DNA synthesis involved in DNA repair strand displacement DNA repair DNA recombination mitotic recombination meiotic nuclear division reciprocal meiotic recombination response to toxic substance response to X-ray replication fork processing strand invasion response to drug protein homooligomerization cellular response to gamma radiation cellular response to cisplatin response to etoposide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RAD51 Monoclonal Antibody (3C10)

RAD51 Antibody (35-6500) in WB

Product Details

35-6500

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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