|Tested species reactivity||Human|
|Host / Isotype||E.coli|
|Storage buffer||tris HCl, pH 8, with 0.01M KCl, 10% glycerol, 1mM DTT, 0.5mM EDTA|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RP-4964 has been successfully used in Western blot proceedures. It is specific for the ~35 kDa Rad51 protein.
The Human gene Rad51 is homologous to the highly conserved bacterial gene RecA.
RP-4964 is highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein. The tag was removed from the recombinant protein (though it still contains Gly-Ser-His at the N-terminal). The protein has been shown to retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52. RP-4964 was confirmed to possess single strand DNA stimulated ATPase activity.
Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. Rad51 functionally and physically interacts with its paralogs Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1, BRCA2, and P53 for the maintenance of genome stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.