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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Human Rad52 recombinant protein expressed in E. coli.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-23334 detects Rad52 in Human samples.
MA1-23334 has been successfully used in Western Blot procedures.
MA1-23334 immunogen corresponds to Human Rad52 recombinant protein expressed in E. coli..
DNA double-strand breaks can be generated by exposure to ionizing radiation or through endogenous cellular reactions. Double-strand breaks are generally thought to be repaired via one of two distinct mechanisms: homologous recombination (HR) or non-homologous end joining (NHEJ). Homologous recombination requires the activity of the members of the RAD52 epistasis group, which includeRAD50, RAD51, RAD52, RAD54, RDH54/TID1, RAD55, RAD57, RAD59, MRE11, and XRS2. RAD52 can catalyze strand annealing reactions, and can form oligomers that attach to ends of single-stranded DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23