|Tested species reactivity||Human|
|Host / Isotype||E.coli|
|Storage buffer||0.02M potassium phosphate, pH 7.5, with 0.2M KCl, 20% glycerol, 2mM beta mercaptoethanol, 0.5mM EDTA|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Control (Ctrl)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RP-4973 contains highly purified recombinant Rad52 protein expressed in E. coli.
RP-4973 may be used as a control in Western blotting applications.
Human Rad52 protein plays a major role in genetic recombination and recombination repair by mediating strand-annealing reaction between complementary DNA strands. Rad52 functionally and physically interacts with Rad51, Dmc1, and RPA in recombination processes. In radiation damaged cell, Rad52 is phosphorylated at Tyr104 by c-Abl tyrosine kinase and forms nuclear foci.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.