OriGene

RAD9A Monoclonal Antibody (OTI7B11), TrueMAB™

View all (33) RAD9 antibodies

Questions & Answers

Questions & Answers

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RAD9A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500434, Dilution 1:50)

RAD9A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RAD9A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500434, Dilution 1:50)

RAD9A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500434, Dilution 1:50)

RAD9A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500434, Dilution 1:50)

RAD9A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-RAD9Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500434, Dilution 1:50)

RAD9A Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAD9A (RC204439, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RAD9A. Positive lysates LY401451 (100 µg) and LC401451 (20 µg) can be purchased separately from OriGene.

RAD9A Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-anti-RAD9Amonoclonal antibody.

Product Details

TA500434

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	OTI7B11
Immunogen	Full-length protein expressed in 293T cell transfected with human RAD9A expression vector View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

RAD9 is a component of the 9-1-1 cell cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesions upon damage by the RAD17-replication factor C clamp loader complex. RAD9 acts as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9; RAD9 homolog A

Gene Aliases: RAD9; RAD9A

UniProt ID: (Human) Q99638

Entrez Gene ID: (Human) 5883

Molecular Function: exodeoxyribonuclease

DNA replication checkpoint DNA damage checkpoint DNA replication DNA repair cellular response to DNA damage stimulus intra-S DNA damage checkpoint cellular response to ionizing radiation nucleic acid phosphodiester bond hydrolysis regulation of signal transduction by p53 class mediator positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage

Process(es)

protein binding 3'-5' exonuclease activity exodeoxyribonuclease III activity SH3 domain binding enzyme binding protein kinase binding histone deacetylase binding