|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rat / IgG1|
|Immunogen||Synthetic peptide GQRDS*SYY*WEIEAS coupled to glutaraldehyde|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-90087 detects RAF1 from human samples.
MA1-90087 has been successfully used in Western blot applications.
The MA1-90087 immunogen is: Synthetic peptide GQRDS*SYY*WEIEAS coupled to glutaraldehyde
This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions.
MA1-90087 was used in immunocytochemistry and western blot to study the expression and distribution of three secretory carrier membrane proteins
|Singleton DR,Wu TT,Castle JD||Journal of cell science (110 ( Pt 17):2099)||1997|