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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 23–342 of human sRAGE|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/ml X 10^6 cells|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chimpanzee, equine, non-human primate and porcine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The receptor for advanced glycation end products (RAGE) is involved in eliciting oxidative stress that results in inflammatory and thrombogenic responses when activated by its ligand, advanced glycation end products (AGE). RAGE is encoded by the class III region of the major histocompatibility complex, which is expressed at sites of inflammation, particularly on inflammatory and epithelial cells. RAGE exists in either membrane bound or as a soluble form (sRAGE) lacking the transmembrane and intracytoplasmic domains. sRAGE also consists of alternate forms including endogenous secretory RAGE (esRAGE). These complexes can bind ligands like AGE and antagonize RAGE signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
RAGE isoform NtRAGE-delta; RAGE isoform sRAGE-delta; receptor for advanced glycation end-products variant 20; sRAGE
AGER; RAGE; SCARJ1