Western blot analysis of RAGE in whole cell extracts from HepG2 (lane 1), Jurkat (lane2), and MDAMB-231 (lane3) cells using a RAGE recombinant rabbit oligoclonal antibody (Product # 710339) at a dilution of 2ug/ml. Samples were detected using chemiluminescence (ECL). Results show a band at ~32kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 23–342 of human sRAGE|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 ug/ml|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The receptor for advanced glycation end products (RAGE) is involved in eliciting oxidative stress that results in inflammatory and thrombogenic responses when activated by its ligand, advanced glycation end products (AGE). RAGE is encoded by the class III region of the major histocompatibility complex, which is expressed at sites of inflammation, particularly on inflammatory and epithelial cells. RAGE exists in either membrane bound or as a soluble form (sRAGE) lacking the transmembrane and intracytoplasmic domains. sRAGE also consists of alternate forms including endogenous secretory RAGE (esRAGE). These complexes can bind ligands like AGE and antagonize RAGE signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.