Immunofluorescence analysis of ABfinity™ RANBP3 Antibody (B24H15L41), Recombinant Rabbit Monoclonal Antibody was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ABfinity™ RANBP3 (B24H15L41) Recombinant Rabbit Monoclonal Antibody (700076) at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with 4ug/ml Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 (A10040) for 45 minutes at room temperature (Panel a: red). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: green) was stained with Alexa Fluor® 488 Phalloidin (A12379, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 151-228|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Ran binding protein 3 (RanBP3) is a member of Ran GTPase binding proteins that is located at the nuclear pore complex (NPC) and plays an essential role in the transport between cytoplasm and nucleus. RANPB3 is expessed as two spliced forms, RanBP3a and RanBP3b, both of which contains two FXFG motifs, characteristic of a subgroup of nucleoporins in addition to nuclear localization signal in the amino-terminal region (amino acid residues 40-57) and a unique acidic carboxyl terminus which is integral to its switch function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AKT activation drives the nuclear localization of CSE1L and a pro-oncogenic transcriptional activation in ovarian cancer cells.
700076 was used in immunohistochemistry to study the role of the AKT pathway in the nuclear localization of CSE1L and the significance for ovarian cancer cell oncogenesis
|Lorenzato A,Biolatti M,Delogu G,Capobianco G,Farace C,Dessole S,Cossu A,Tanda F,Madeddu R,Olivero M,Di Renzo MF||Experimental cell research (319:2627)||2013|