|Tested species reactivity||Human, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A mixture of two peptides from Xenopus RanGAP, n-CHWSDMFTGRLRPEI-c n-CPSPEKLVRMGPRRSA-|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-5866 detects RanGAP in human and xenopus samples.
PA1-5866 has been successfully used in immunofluorescence, immunohistochemistry (paraffin) and Western blot procedures.
The PA1-5866 immunogen is two peptides: n-CHWSDMFTGRLRPEI-c and n-CPSPEKLVRMGPRRSA-c, from Xenopus RanGAP.
Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.