|Tested species reactivity||Human, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||His-tagged recombinant Xenopus RCC1 protein purified from E. coli.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-5868 detects RanGEF in human and xenopus samples.
PA1-5868 has been successfully used in immunofluorescence, immunoprecipitation, immunohistochistry and Western blot procedures.
The PA1-5868 immunogen is His-tagged recombinant Xenopus RCC1 protein purified from E. coli.
GTP exchange factor, RCC1, with chromatin , but Ran is shown here to bind directly to chromatin in two modes, either dependent or independent of RCC1, and, where bound, to increase the affinity of chromatin for NE membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.