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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human RANTES|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
P230E targets RANTES in ELISA, and WB applications and shows reactivity with Human samples.
The P230E immunogen is recombinant human RANTES.
P230E detects RANTES which has a predicted molecular weight of approximately 8 kDa.
The P230E Rantes polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M230B (biotinylated conjugate of clone VL-1) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody P230E and biotinylated antibody M230B (clone VL-1) have successfully been used in combination with recombinant Rantes protein SRNTS in ELISA applications.
This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Human immunodeficiency virus type 1 viral protein R (Vpr) induces CCL5 expression in astrocytes via PI3K and MAPK signaling pathways.
P230E was used in immunocytochemistry to study the role of PI3 kinase and MAPK in the mechanism by which the HIV1 viral protein R induces astrocyte CCL5 expression
|Gangwani MR,Noel RJ,Shah A,Rivera-Amill V,Kumar A||Journal of neuroinflammation (10:null)||2014|
Dengue virus selectively induces human mast cell chemokine production.
P230E was used in ELISA to study the induction of human mast cell chemokine production by Dengue virus
|King CA,Anderson R,Marshall JS||Journal of virology (76:8408)||2002|
beta-chemokine RANTES; chemokine (C-C motif) ligand 5; eoCP; eosinophil chemotactic cytokine; regulated upon activation, normally T-expressed, and presumably secreted; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; small-inducible cytokine A5; t cell-specific protein P228; T-cell specific protein p288
CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228