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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human RANTES.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5 - 2.0 ug/ml|
|Functional Assay (FN)||5.0 - 7.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||0.1-0.2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. This antibody may be used in Western Blotting applications under either reducing or non-reducing conditions.
Reconstitute with 0.1 ml of distilled water. For long term storage the addition of 0.09% sodium azide is recommended. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
beta-chemokine RANTES; chemokine (C-C motif) ligand 5; D17S136E; eosinophil chemotactic cytokine; MGC17164; RANTES; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SISd; small inducible cytokine subfamily A (Cys-Cys), member 5; T-cell specific protein p288; TCP228
CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228