|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human RANTES|
|Storage buffer||PBS with 4% BSA|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
M230B targets RANTES in ELISA, and WB applications and shows reactivity with Human samples.
The M230B immunogen is recombinant human RANTES.
M230B detects RANTES which has a predicted molecular weight of approximately 8 kDa.
The M230B Rantes antibody (biotinylated conjugate of clone VL-1) has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody P230E. Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Biotinylated antibody M230B (clone VL-1) and antibody P230E have successfully been used in combination with recombinant Rantes protein SRNTS in ELISA applications.
This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Dose dependent effects of platelet derived chondroitinsulfate A on the binding of CCL5 to endothelial cells.
M230B was used in flow cytometry to assess the impact of platelet derived glycosaminoglycans on the immobilization of CCL5 on human microvascular endothelial cells
|Weingart C,Nelson PJ,Krämer BK,Mack M||BMC immunology (9:null)||2008|