|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 212-235 of human Rap1.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: 293 whole cell lysate, antigen standard for TERF2IP (transient overexpression lysate).
Human Rap1 was the first identified ortholog of the yeast telomeric protein scRap1, which shares 3 conserved sequence motifs in common. HRap1 is located at telomeres and affects telomere length by being recruited to telomeres by TRF2 instead of directly binding to the telomere like scRap1. The hRap1 cDNA codes for a protein of 399 amino acids with approximate molecular weight of 47 kDa. The hRap1 protein does not interact with TRF1. TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length(1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (5). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (4). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (4). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.