|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human RAP1A expressed in E. Coli.|
|Storage buffer||PBS with 0.5% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17164 targets RAP1A in FACS, pep-ELISA, and WB applications and shows reactivity with Human samples.
The MA5-17164 immunogen is purified recombinant fragment of human RAP1A expressed in E. Coli.
MA5-17164 detects RAP1A which has a predicted molecular weight of approximately 21kDa.
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.