Invitrogen

RAR gamma-1,2 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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RAR gamma-1,2 Antibody in Western Blot (WB) — Western Blot analysis of RAR gamma 1, 2 in various cell lines using a RAR gamma 1, 2 Polyclonal antibody (Product # PA5-121101) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.

Product Details

PA5-121101

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence within amino acids 144-223 of human RARG (NP_000957.1). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.69 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.01% thimerosal
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2914673

Product Specific Information

Positive test controls include: Mouse lung, Rat kidney. The target is usually found in the following locations: Nucleus.

Target Information

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD. The corresponding gene for the retinoic acid receptor gamma 1 is NR1B3.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Nuclear receptor subfamily 1 group B member 3; RAR gamma 2; RAR-gamma; retinoic acid nuclear receptor gamma variant 1; retinoic acid nuclear receptor gamma variant 2; Retinoic acid receptor gamma; retinoic acid receptor gamma 4; retinoic acid receptor, gamma

Gene Aliases: NR1B3; RAR-gamma; RARC; RARD; RARG; RARgamma2

UniProt ID: (Human) P13631, (Mouse) P18911

Entrez Gene ID: (Human) 5916, (Mouse) 19411, (Rat) 685072

Molecular Function: C4 zinc finger nuclear receptor