Invitrogen

RAR gamma-1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols
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Product Details

PA3-813

Applications
Tested Dilution
Publications

Western Blot (WB)

1:250-1:500
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent
-

Gel Shift (GS)

1-2 µL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the residues E(40) M L S P S F R G L G Q P D L P K E(57) of the N-terminal end of RAR gamma-1.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2253413

Product Specific Information

PA3-813 detects recombinant human retinoic acid receptor (RAR) gamma-1. The immunizing sequence is 100% conserved in mouse. This antibody detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.

PA3-813 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RAR gamma-1 from transiently transfected 293T cells. PA3-813 has been shown to efficiently super shift the human RAR gamma-1/retinoid X receptor (RXR)/RAR response element complex in gel super shift experiments using a number of different response elements.

PA3-813 immunizing peptide corresponds to the N-terminus of RAR gamma-1. This peptide (Cat. # PEP-228) is available for use in neutralization and control experiments.

Target Information

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma. Two main isoforms of RAR gamma exist: RAR gamma-1 has been found to be predominantly expressed in skin, while RAR gamma-2 has been found early in embryogenesis and in embryonal carcinoma cells. RAR gamma is also known as RARD. The corresponding gene for the retinoic acid receptor gamma 1 is NR1B3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 1 group B member 3; RAR gamma 2; RAR Gamma-1; RAR-gamma; retinoic acid; retinoic acid nuclear receptor gamma variant 1; retinoic acid nuclear receptor gamma variant 2; retinoic acid receptor; Retinoic acid receptor gamma; retinoic acid receptor gamma 4; retinoic acid receptor, gamma

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Gene Aliases: NR1B3; RAR-gamma; RARC; RARD; RARG; RARgamma2

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UniProt ID: (Human) P13631, (Mouse) P18911

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Entrez Gene ID: (Human) 5916, (Mouse) 19411, (Rat) 685072

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity retinoic acid receptor activity protein binding zinc ion binding retinoid X receptor binding sequence-specific DNA binding metal ion binding C4 zinc finger nuclear receptor
Process(es)
negative regulation of transcription from RNA polymerase II promoter neural tube closure glandular epithelial cell development retinal pigment epithelium development growth plate cartilage chondrocyte growth transcription initiation from RNA polymerase II promoter positive regulation of cell proliferation negative regulation of cell proliferation regulation of cell size anterior/posterior pattern specification embryonic camera-type eye development regulation of myelination negative regulation of chondrocyte differentiation response to retinoic acid embryonic hindlimb morphogenesis multicellular organism growth positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death steroid hormone mediated signaling pathway regulation of myeloid cell differentiation positive regulation of transcription from RNA polymerase II promoter embryonic eye morphogenesis retinoic acid receptor signaling pathway canonical Wnt signaling pathway face development trachea cartilage development prostate gland epithelium morphogenesis Harderian gland development cellular response to retinoic acid chondrocyte development growth plate cartilage development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter regulation of gene expression positive regulation of gene expression camera-type eye development negative regulation of cell differentiation reproductive structure development gland development retina development in camera-type eye limb development bone development bone morphogenesis epithelium development negative regulation of cartilage development
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