Invitrogen

RASSF1A Monoclonal Antibody (eB114-10H1), eBioscience™

15 Published Figures

20 References

View all (24) RASSF1 antibodies

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Datasheet

Questions & Answers

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RASSF1A Antibody — Fig. 5 RASSF1A suppresses PI3K-Akt-mTOR pathway to promote autophagy initiation through Hippo pathway regulatory protein MST1. a Representative immunoblots showing the fragment of RASSF1A coimmunoprecipitated with MST1 in 293T cells. b Representative immunoblots showing the impact of fragments of RASSF1A on the amount of MST1 coimmunoprecipitated with RASSF1A in 293T cells. Inputs are the same as in panel a . c , d Representative immunoblots ( c ) and quantification showing the impact of RASSF1A on levels of total and p-MST1 ( d ) in mouse liver tissues. e , f Representative immunoblots ( e ) and quantification ( f ) showing the impact of RASSF1A on levels of p-YAP, YAP, CTGF and Beclin 1 in mouse liver tissues. g , h Representative immunoblots showing levels of MST1 and Akt coimmunoprecipitated with RASSF1A ( g ) or levels of MST1 and RASSF1A coimmunoprecipitated with Akt ( h ) in HeLa cells. I , j Representative immunoblots ( i ) and quantification showing the impact of RASSF1A suppression on the levels of total MST1 or MST1 coimmunoprecipitated (IP-MST1) with Akt ( j ) in HeLa cells treated with random (Mock) or RASSF1A-specific siRNAs. k - r Representative immunoblots ( k , m , o , q ) and quantification showing the levels of p-AKT and p-S70S6K ( l , n , p , r ) in mouse liver tissues ( k , l ), MEFs ( m , n ), liver tissues from DEN-treated 6-month-old mice ( o , p ), or HeLa cells treated with random (Mock) or RASSF1A-specific siRNAs ( q , r

Product Details

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

Assay-Dependent

View 3 publications 3 publications

Western Blot (WB)

1-10 µg/mL

View 13 publications 13 publications

Immunocytochemistry (ICC)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunofluorescence (IF)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Artificial Control, Human, Mouse

Expression System

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eB114-10H1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_468373

Product Specific Information

Description: The eB114 antibody reacts with the RASSF1A isoform of the tumor suppressor gene RASSF1. RASSF1A contains a Ras Association Domain (RA) and binds Ras in a GTP-dependent manner to effect apoptosis. The RASSF1A isoform undergoes epigenetic inactivation in a variety of human primary tumors. This has been associated with hypermethylation of a CpG island(s) in its promoter region. This antibody was generated against a synthetic peptide corresponding to the splice junction region of RASSF1A.

Applications Reported: The eB114 antibody has been reported for use in immunoprecipitation, and immunoblotting (WB).

Applications Tested: The eB114-10H1 has been tested by Western blotting and can be used at approximately 1-10 µg/mL. RASS1A is detected as a 45 kDa band in Western blotting of a HeLa cell extract. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Seven alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cardiac-specific ras association domain family 1 protein; pancreas-specific ras association domain family 1 protein; Ras association (RalGDS/AF-6) domain family member 1; Ras association domain-containing protein 1; RASSF1; tumor suppressor protein RDA32; WUGSC:H_LUCA12.5

Gene Aliases: 123F2; NORE2A; RASSF1; RASSF1A; RDA32; REH3P21

UniProt ID: (Human) Q9NS23

Entrez Gene ID: (Human) 11186

Molecular Function: G-protein modulator enzyme modulator