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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:500 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
3H3F12 |
Immunogen |
RBM4 Fusion Protein Ag2190 (1-177 aa encoded by BC021120) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.45 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MVKLFIGNL PREATEQEIR SLFEQYGKVL ECDIIKNYGF VHIEDKTAAE DAIRNLHHYK LHGVNINVEA SKNKSKTSTK LHVGNISPTC TNKELRAKFE EYGPVIECDI VKDYAFVHME RAEDAVEAIR GLDNTEFQGE PPSLGRGLNT RLCAENGWIS KRRGLVKITA VGWLVMKK (1-177 aa encoded by BC021120)
RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hLark; Lark homolog; rna-binding; RNA-binding motif protein 4; RNA-binding motif protein 4a; RNA-binding protein 4; transcriptional coactivator CoAZ; zinc finger CCHC-type and RNA binding motif 3A
Gene Aliases: LARK; RBM4; RBM4A; ZCCHC21; ZCRB3A
UniProt ID: (Human) Q9BWF3
Entrez Gene ID: (Human) 5936
Molecular Function:
RNA splicing factor
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