Immunofluorescent analysis of RBX1 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RBX1 polyclonal antibody (Product # PA5-29149) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 44 and 108 of Human RBX1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29149 targets RBX1 in WB applications and shows reactivity with mouse samples.
The PA5-29149 immunogen is synthetic peptide corresponding to a region within amino acids 44 and 108 of Human RBX1.
This gene encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.
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