|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 95 and 352 of Human RENT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-29480 targets RENT1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29480 immunogen is recombinant fragment corresponding to a region within amino acids 95 and 352 of Human RENT1.
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP-dependent helicase RENT1; delta helicase; HUPF1; nonsense mediated mRNA decay factor; Nonsense mRNA reducing factor 1; NORF1; pNORF1; Regulator of nonsense transcripts 1; RENT1; smg-2; smg-2 homolog; smg-2 homolog, nonsense mediated mRNA decay factor; UP Frameshift 1; up-frameshift mutation 1 homolog; Up-frameshift suppressor 1 homolog; UPF1 regulator of nonsense transcripts homolog; yeast Up; yeast Upf1p homolog
Gene Aliases: HUPF1; KIAA0221; NORF1; pNORF1; RENT1; smg-2; UPF1
UniProt ID: (Human) Q92900
Entrez Gene ID: (Human) 5976
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.