Invitrogen

RFP Polyclonal Antibody

Product Image Gallery

RFP Antibody — FIGURE 6 Transmission electron micrographs showing immunogold labeling of N. benthamiana peroxisomes in cells co-expressing PTS2-DsRed together with either GFP-CHEC89 (A,B) or GFP-ChEC96 (C-F) . In each case, adjacent ultrathin sections through the same peroxisome were probed with either anti-GFP antibodies to label the GFP-tagged ChECs or anti-RFP antibodies to label the peroxisome matrix. All micrographs are presented at the same magnification, bar = 500 nm.

RFP Antibody in Immunofluorescence (IF) — HeLa cells were incubated with Organelle Lights™ Mito-RFP (O36229) and Organelle Lights™ Golgi-GFP (O10098). Following fixation and permeabilization, cells were stained with Alexa Fluor® 350 phalloidin (Product # A22281) and anti-RFP rabbit polyclonal antibody (Product # R10367), the latter subsequently visualized with Alexa Fluor® 647 Goat Anti-Rabbit IgG antibody (Product # A-21245, red fluorescence) and imaged. No cross-reactivity is observed of the anti-RFP antibody with GFP (green-fluorescence, perinuclear region).

RFP Antibody in Western Blot (WB) — Specificity of anti-RFP on western blot. 10 mg of protein lysates from U-2 OS cells expressing Cellular Lights™ Histone 2B-RFP (lane 1, C10128) or Histone 2B-GFP (lane 2, C10129). Anti-RFP antibody detects the Cellular Lights™ Histone 2B-RFP (~ 41 kDa) but not Histone 2B-GFP.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:10,000-1:15,000

Immunocytochemistry (ICC)

1:5000

Immunofluorescence (IF)

1:5000

Western Blot (WB)

1:3000-1:7000

Published Applications

Immunomicroscopy (IM)

See 2 publications below

Immunocytochemistry (ICC)

See 7 publications below

Immunofluorescence (IF)

See 13 publications below

Immunohistochemistry (IHC)

See 10 publications below

Western Blot (WB)

See 12 publications below

Immunoprecipitation (IP)

See 2 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Miscellaneous PubMed (MISC)

See 3 publications below

Product Specifications

Species Reactivity

Tag

Published species

Fruit fly, Human, Mouse, Tag

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full-length recombinant denatured and non-denatured TagRFP was used as the immunogen

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 0.25mg/mL gelatin, 1% trehalose

Contains

no preservative

Storage conditions

-20° C, store in dark

RRID

AB_10563941

Product Specific Information

This antibody can be used to detect native RFP, RFP variants, and RFP fusion proteins in Western blot, immunoprecipitation, ELISA, and immunocytochemistry. This antibody has been shown to detect denatured and native TurboRFP, TurboFP602, TurboFP635, TagRFP, TagFP635, and mKate2.

Storage and reconstitution: to prepare a 1 mg/mL stock solution, add 100 µl sterile water or 50% glycerol to the vial. If desired, aliquot the antibody upon reconstitution to produce single-use samples. Store the reconstituted product with sterile water at 4°C, avoiding freeze/thaw cycles. When stored as directed, the solution is stable for 3 months. If reconstituted with 50% glycerol, store at -20°C, avoiding freeze/thaw cycles. When stored as directed, this solution is stable for 6 months.

Target Information

Red Fluorescent Protein (RPF) is isolated from sea anemone (Discosoma sp), and commonly used as a molecular tag to study protein localization, reporter gene expression, and protein-protein interactions. Although it exists as a homotetramer, monomeric variants have been constructed for use in various applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Red fluorescent protein; RFP tag

Suggested Secondary Antibodies

Anti-Rabbit Secondary Antibodies (587)
Alexa Fluor® Anti-Rabbit Secondary Antibodies (130)
HRP Anti-Rabbit Secondary Antibodies (80)

RFP Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Suggested Secondary Antibodies

Material safety data sheets (MSDS)

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