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ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the anti-RFXAP antibody (Cat. no. 49-1034), and optimized primer sets. Each ChIP assay used sheared chromatin from 2 million cells and 5 µg (lane 3) and 7 µg (lane 4) of anti-RFXAP antibody. An anti-flag antibody (lane 2) is used as negative IP control. The HLA_DRA primer pair was used as positive PCR control, and HLA_DOB was used a negative PCR control. The ChIP results using the anti-RFXAP antibody (Cat. no. 49-1034) show a clear signal for the positive primer pair (HLA_DRA) with respectively 5 and 7 µg of antibody per IP (lower panel, lanes 3 and 4) and show no signal for the negative primer pair (HLA_DOB) (upper panel, lanes 3 and 4). The input is shown in lane 1 and the negative IP control (anti-flag antibody) is shown in lane 2.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against the recombinant RFXAP protein.|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5-7 ug|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulatory factor X-associated protein; RFX DNA-binding complex 36 kDa subunit; RFX-AP; RFX-associated protein