|Tested species reactivity||Bovine, Mouse|
|Published species reactivity||Bovine|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length, bacterially expressed RGS9-1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-723 detects RGS9-1 protein from bovine and mouse samples.
MA1-723 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~57 kDa protein representing RGS-1 protein from bovine retinal extracts.
The MA1-723 immunogen is full length, bacterially expressed RGS9-1 protein.
The cascade of biochemical reactions within the vision system is complex and contains numerous steps taking place in nanoseconds. RGS is a family of GTPase accelerating proteins (GAPs). RGS9 is a member of this family and is the predominant source of GAP activity in rod outer segments. RGS9-1, found predominately in vertebrate cone and rod photoreceptors, is a useful model for understanding how rapid recovery kinetics of the light responses in retinal photoreceptors take place.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dependence of RGS9-1 membrane attachment on its C-terminal tail.
MA1-723 was used in western blot to investigate membrane attachment of RGS9-1
|He W,Melia TJ,Cowan CW,Wensel TG||The Journal of biological chemistry (276:48961)||2001|
High expression levels in cones of RGS9, the predominant GTPase accelerating protein of rods.
MA1-723 was used in immunocytochemistry to study the expression of RGS9 in cones
|Cowan CW,Fariss RN,Sokal I,Palczewski K,Wensel TG||Proceedings of the National Academy of Sciences of the United States of America (95:5351)||1998|