|Tested species reactivity||Human, Mouse, Non-human primate|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide (31 PWFREEEVQYIQAEKNNKG 49) of RIG-I/DDx58 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
RIG-I, an RNA helicase of the DEAD/H box family, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), is implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure and in innate immune defense against viruses. It acts as a specific sensor for dsRNA, which is absent in uninfected cells but known to be accumulated in virus-infected cells. It contains two CARD domains, one helicase ATP-binding domain and the other helicase C-terminal domain. The CARD domains actually inhibit the activity of RIG-I in normal cell, but once the cell gets infected, RIG I directly binds uncapped 5-prime-triphosphate RNA present in viruses triggering an immune response signal. The signaling cascade involves MAVS/IPS1 which induces the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5) during viral replication through activation of NF-kappa-B, IRF3 and IRF7. The ability of RIG I to sense 5-prime-phosphorylated RNA evolved in the innate immune system as a means of discriminating between self and nonself.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Dermatomyositis With or Without Anti-Melanoma Differentiation-Associated Gene 5 Antibodies: Common Interferon Signature but Distinct NOS2 Expression.
MA5-16254 was used in immunohistochemistry - frozen section to characterize dermatomyositis with or without anti-melanoma differentiation-associated gene 5 antibodies that show distinct NOS2 expression but a common interferon signature
|Allenbach Y,Leroux G,Suárez-Calvet X,Preusse C,Gallardo E,Hervier B,Rigolet A,Hie M,Pehl D,Limal N,Hufnagl P,Zerbe N,Meyer A,Aouizerate J,Uzunhan Y,Maisonobe T,Goebel HH,Benveniste O,Stenzel W||The American journal of pathology (186:691)||2016|