Western blot detection of RING1B in 20µg NIH-3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Samples were run on a SDS-PAGE, transferred onto nitrocellulose, and probed using a RING1B polyclonal antibody (Product # PA1-31795) at a dilution of 1:500 following incubation at room temperature. Signal was detected using standard techniques.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide EAGPSNKRTKTSDC, corresponding to amino acids 189-201 of Human RING1B.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31795 detects RING1B from human samples.
PA1-31795 has been successfully used in Western blot applications.
The PA1-31795 immunogen is: Synthetic peptide: EAGPSNKRTKTSDC, corresponding to amino acids 189-201 of Human RING1B.
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.