Description: This rabbit polyclonal antibody reacts with mouse and rat Receptor Interacting Protein (RIP) 3. This 57 kDa Ser/Thr kinase is ubiquitously expressed and resides primarily within the cytoplasm. However, studies have suggested that this protein can shuttle between the nucleus and cytoplasm. Although its kinase domain is similar to that of RIP1 and 2, RIP3 does not possess a death domain or CARD motif. RIP3 binds to and phosphorylates RIP1, resulting in inhibition of RIP1- and TNFR1-mediated NFkB activation. RIP3 mediates programmed necrosis by regulating TNF-induced reactive oxygen species production. Moreover, studies have also identified RIP3 as the molecular switch between TNF-induced apoptosis and necrosis in some cell types.
Applications Reported: This polyclonal antibody has been reported for use in western blotting and immunohistochemical staining.
Applications Tested: This polyclonal antibody has been tested by western blot analysis of NIH-3T3 cell lysate. This can be used at 0.5-4 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
RIP3 is a novel member of RIP Ser/Thr kinase family. It is a potent inducer of apoptosis and is an important component of TNFR1 signaling complex. RIP3 consists of an N-terminal RIP-like-Kinase domain and a unique, non-homologous C-terminus responsible for caspase activation and apoptosis induction. RIP3 is expressed in multiple tissues including hematopoietic cells and plays a functional role in the regulation of apoptosis and NF-kB signaling. It is recruited to the TNFR1 signaling complex in a RIP-dependant manner where it induces apoptosis by activating caspases and/or inhibiting TNFR1-induced NF-kB activation. RIP3 negatively regulates the TLR3-Trif-mediated NF-kB signaling pathway by competing with the binding of Trif to RIP1. The human RIP3 gene is localized in the chromosomal region 14q11.2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Homocysteine respondent protein HCYP2; mRIP3; receptor interacting protein 3; Receptor-interacting protein 3; Receptor-interacting serine/threonine-protein kinase 3; RIP-3; RIP-like protein kinase 3
Gene Aliases: 2610528K09Rik; AW107945; Hcyp2; Rip3; Ripk3