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RNF149 Antibody (CF810423) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF149 (RC207979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF14. Positive lysates LY406531 (100 µg) and LC406531 (20 µg) can be purchased separately from OriGene.
Product Details
CF810423
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI10B3
Immunogen
Human recombinant protein fragment corresponding to amino acids 250-383 of human RNF149 produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
RNF149 is a E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase-transactivated protein 2; E3 ubiquitin-protein ligase RNF149; RING finger protein 149; RING-type E3 ubiquitin transferase RNF149
Gene Aliases: DNAPTP2; RNF149
UniProt ID: (Human) Q8NC42
Entrez Gene ID: (Human) 284996
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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