Western blot analysis of ROR alpha in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse skeletal muscle lysate and 4) rat skeletal muscle lysate using a ROR alpha polyclonal antibody (Product # PA5-23268) at 0.25 ug/ml, 0.01 ug/ml and 0.01 ug/ml, respectively.
|Tested species reactivity||Bovine, Dog, Chicken, Horse, Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (94 SKKQRDSLYAEVQKHR 109) of ROR alpha protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.2ml PBS, pH 8.0, with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.01-0.25 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
RORA is a receptor for retinoic acid belonging to the NR1 subfamily of nuclear hormone receptors with a nuclear receptor DNA binding domain. RORA binds either as a monomer or as a homodimer to the retinoic acid response element and thus regulates gene expression and also controls cell function. Though the exact function of RORA is yet unknown, it interacts with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, NM23-1, the product of a tumor metastasis suppressor candidate gene, CDK7, NCOA3 and NCOA6 coactivators. It is found to express in mesenchymal stem cells derived from bone marrow and are known to act by direct modulation of a bone matrix component. It is widely expressed in most of the tissues. Chromosomal aberrations involving RORA may be a cause of acute promyelocytic leukemia (APL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.