Invitrogen

ROR gamma (t) Monoclonal Antibody (B2D), eBioscience™

Product Image Gallery

ROR gamma (t) Antibody — Published figure using ROR gamma (t) monoclonal antibody (Product # 14-6981-82)

ROR gamma (t) Antibody in Western Blot (WB) — Left: Mouse thymus (left) and spleen (right) nuclear cell lysates were loaded at 1x10e5 cells/lane, probed with 2 µg/mL of Anti-Mouse ROR gamma (t) Purified and revealed with Anti-Rat IgG HRP. Right: Untransfected (left) and mouse ROR gamma-transfected (right) 293T nuclear cell lysates were loaded at 1 x 10e5 cells/lane, probed with 2 µg/mL of Anti-Mouse ROR gamma (t) Purified and revealed with HRP anti-rat IgG. Note: ROR gamma runs at a slightly higher molecular weight in the transfected cell lysate, compared to the thymus lysate, due to the addition of a tag to full length ROR gamma in the transfected cells.

Product Details

Tested Applications

Dilution

Western Blot (WB)

2 µg/mL

Published Applications

Flow Cytometry (Flow)

See 17 publications below

Miscellaneous PubMed (MISC)

See 5 publications below

Western Blot (WB)

See 14 publications below

Immunofluorescence (IF)

See 2 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 2 publications below

Immunohistochemistry (IHC)

See 1 publication below

Gel Shift (GS)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B2D

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_925759

Product Specific Information

Description: The B2D monoclonal antibody reacts with the mouse RORgamma(t) protein. RORgamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes RORalpha and RORbeta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. RORgamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, RORgammat, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs.

The RORgammat isoform differs from RORgamma by three unique amino acids at the amino terminus. Therefore, the B2D antibody will react with both the RORgamma and RORgammat isoforms.

Applications Reported: This B2D antibody has been reported for use in immunoblotting (WB).

Applications Tested: This B2D antibody has been tested by western blot analysis of mouse thymus nuclear lysates. This B2D antibody can be used at a starting concentration of 2 µg/mL, however the optimal concentration should be determined for individual experimental conditions.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma t, a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: MGC129539; NR1F3; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; Retinoid-related orphan receptor-gamma; RORG; RORgt; RZR-GAMMA; RZRG; Thymus orphan receptor; TOR

Gene Aliases: Nr1f3; Rorc; Rorg; RORgamma; Thor; TOR

UniProt ID: (Mouse) P51450

Entrez Gene ID: (Mouse) 19885

Molecular Function: nuclear hormone receptor nucleic acid binding receptor transcription factor