|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Clone||2H10 (70C or alphaSSB70C)|
|Immunogen||Purified human RPA protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Gel Shift (GS)||1mg/mL|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13953 targets RPA/p70 in GS, IP, and WB applications and shows reactivity with Human samples.
The MA5-13953 immunogen is purified human RPA protein.
Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) is involved in DNA replication, repair, and recombination. RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA70 is composed of three domains: an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ATR signaling can drive cells into senescence in the absence of DNA breaks.
MA5-13953 was used in immunocytochemistry to investigate the role of ATR signaling in the senescence of cells in the absence of DNA breaks
|Toledo LI,Murga M,Gutierrez-Martinez P,Soria R,Fernandez-Capetillo O||Genes and development (22:297)||2008|
HIV-1 Vpr induces ATM-dependent cellular signal with enhanced homologous recombination.
MA5-13953 was used in immunocytochemistry to study the role of HIV-1 Vpr in viral integration and HIV pathogenesis
|Nakai-Murakami C,Shimura M,Kinomoto M,Takizawa Y,Tokunaga K,Taguchi T,Hoshino S,Miyagawa K,Sata T,Kurumizaka H,Yuo A,Ishizaka Y||Oncogene (26:477)||2007|
A pRb-independent mechanism preserves the postmitotic state in terminally differentiated skeletal muscle cells.
MA5-13953 was used in western blot to study a retinoblastoma protein-independent mechanism for preserving the postmitotic state in skeletal muscle cells
|Camarda G,Siepi F,Pajalunga D,Bernardini C,Rossi R,Montecucco A,Meccia E,Crescenzi M||The Journal of cell biology (167:417)||2004|
A multiprotein nuclear complex connects Fanconi anemia and Bloom syndrome.
MA5-13953 was used in western blot to investigate the role of the multiprotein Fanconi anemia complex in DNA repair
|Meetei AR,Sechi S,Wallisch M,Yang D,Young MK,Joenje H,Hoatlin ME,Wang W||Molecular and cellular biology (23:3417)||2003|
The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene.
MA5-13953 was used in western blot to study the interaction between a replication protein A subunit and MEN1 tumor suppressor gene product
|Sukhodolets KE,Hickman AB,Agarwal SK,Sukhodolets MV,Obungu VH,Novotny EA,Crabtree JS,Chandrasekharappa SC,Collins FS,Spiegel AM,Burns AL,Marx SJ||Molecular and cellular biology (23:493)||2003|
Autoimmune responses to proliferating cell nuclear antigen multiprotein complexes involved in cell proliferation are strongly associated with their structure and biologic function in patients with systemic lupus erythematosus.
MA5-13953 was used in ELISA to study the mechanism of autoimmune responses to PCNA and its associated proteins in systemic lupus erythematosus
|Kogure T,Takasaki Y,Takeuchi K,Yamada H,Nawata M,Ikeda K,Matsushita M,Matsudaira R,Kaneda K,Terasawa K,Hashimoto H||Arthritis and rheumatism (46:2946)||2002|