Invitrogen

RPA2 Monoclonal Antibody (MA34)

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Datasheet

Product Image Gallery

RPA2 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of RPA34 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RPA34 monoclonal antibody (Product # MA1-870) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). RPA34 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

RPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a RPA34 monoclonal antibody (Product # MA1-870) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

RPA2 Antibody in Western Blot (WB) — Knockdown of RPA2 was achieved by transfecting Caco-2 with RPA2 specific siRNAs (Silencer® select Product # s12130, s12132). Western blot analysis (Fig. a) was performed using whole cell extracts from the RPA2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RPA2 Monoclonal Antibody (MA34) (Product # MA1-870, 1:500 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPA2.

RPA2 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. RPA2 cells were transfected with RPA2 siRNA and reduction of signal was observed in Western Blot using RPA2 Monoclonal Antibody (MA34) (Product # MA1-870). {KD}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Western Blot (WB)

1:500

Product Specifications

Species Reactivity

Human, Non-human primate, Rat

Host / Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

MA34

Immunogen

RPA p34 subunit isolated from HeLa cells.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_795759

Product Specific Information

MA1-870 detects RPA p34 in human and rat cells.

MA1-870 has been successfully used in Western blotting, immunofluorescence and immunohistochemistry (paraffin) procedures. By Western blot it detects a 34 kDa protein representing RPA p34.

The MA1-870 immunogen is the RPA p34 subunit isolated from HeLa cells.

Target Information

RPA p34 is a subunit of replication protein A, also known as Human single-stranded DNA-binding protein (HSSB). P11 and p34 are thought to form the base for the complex before p70 is added to the complex, as they can form stable complexes together, but neither can form a stable complex with p70.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HSSB; p32-subunit of replication protein A; Replication factor A protein 2; Replication Protein A; Replication protein A 32 kDa subunit; Replication protein A 34 kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RP-A p32; RP-A p34

Gene Aliases: REPA2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34

UniProt ID: (Human) P15927, (Rat) Q63528

Entrez Gene ID: (Human) 6118, (Rat) 59102

Function(s)