OriGene

RPA2 Monoclonal Antibody (OTI9A1), TrueMAB™

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Datasheet

Protocols

Questions & Answers

Tech Support

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RPA2 Antibody in Immunocytochemistry (ICC/IF) — Anti-RPA2 mouse monoclonal antibody (TA500763) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).

RPA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500763)

RPA2 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2. Positive lysates LY401031 (100 µg) and LC401031 (20 µg) can be purchased separately from OriGene.

RPA2 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells, using anti-RPA2 antibody (TA500763), (Red), compared to a nonspecific negative control antibody, (Blue).

RPA2 Antibody in Immunoprecipitation (IP) — Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Product Details

CF500763

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-
Immunoprecipitation (IP)	2 µg/500 µL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI9A1
Immunogen	Full length human recombinant protein of human RPA2 produced in HEK293T cell. View immunogen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HSSB; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A 34kDa subunit; replication protein A2, 32kDa; RF-A protein 2; RFA; RP-A p32; RP-A p34; RP4-547C9.3; RpLP1; RpP2

Gene Aliases: REPA2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34

UniProt ID: (Human) P15927

Entrez Gene ID: (Human) 6118

Function(s)

Process(es)

damaged DNA binding single-stranded DNA binding protein binding enzyme binding protein phosphatase binding ubiquitin protein ligase binding protein N-terminus binding

RPA2 Monoclonal Antibody (OTI9A1), TrueMAB™

Product Details

CF500763

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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