|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length human protein.|
|Storage buffer||PBS with 1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 11 publications below|
|Western Blot (WB)||See 9 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Gel Shift (GS)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
MA1-26418 detects RPA34 from human samples.
MA1-26418 has been successfully used in immunohistochemistry (frozen and paraffin), immunofluorescence, immunocytochemistry, and Western blot procedures.
The MA1-26418 immunogen is full length human protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PC4 promotes genome stability and DNA repair through binding of ssDNA at DNA damage sites.
MA1-26418 was used in immunocytochemistry to investigate the effect of PC4 on genome stability and DNA repair
|Mortusewicz O,Evers B,Helleday T||Oncogene (35:761)||2016|
DNA end resection is needed for the repair of complex lesions in G1-phase human cells.
MA1-26418 was used in immunocytochemistry to study double stranded break repair during different stages of the cell cycle.
|Averbeck NB,Ringel O,Herrlitz M,Jakob B,Durante M,Taucher-Scholz G||Cell cycle (Georgetown, Tex.) (13:2509)||2014|
Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome.
MA1-26418 was used in immunocytochemistry to study the tolerance of human cells to DNA damage resulting from low-dose UV exposure and the roles of replication fork gap-filling and bypass in this tolerance
|Quinet A,Vessoni AT,Rocha CR,Gottifredi V,Biard D,Sarasin A,Menck CF,Stary A||DNA repair (14:27)||2014|
Scaffold functions of 14-3-3 adaptors in B cell immunoglobulin class switch DNA recombination.
MA1-26418 was used in immunocytochemistry to study the role of 14-3-3 adaptor scaffold function in immunoglobulin class switch DNA recombination in B-cells
|Lam T,Thomas LM,White CA,Li G,Pone EJ,Xu Z,Casali P||PloS one (8:null)||2013|
BLM SUMOylation regulates ssDNA accumulation at stalled replication forks.
MA1-26418 was used in immunocytochemistry and western blot to study the mechanism by which SUMOylation of the BLM helicase modulates the accumulation of single-stranded DNA at stalled replication forks
|Ouyang KJ,Yagle MK,Matunis MJ,Ellis NA||Frontiers in genetics (4:null)||2013|
Mammalian Exo1 encodes both structural and catalytic functions that play distinct roles in essential biological processes.
MA1-26418 was used in immunocytochemistry to study the catalytic and structural involvement of mammalian exonuclease-1 in a range of biological processes
|Schaetzlein S,Chahwan R,Avdievich E,Roa S,Wei K,Eoff RL,Sellers RS,Clark AB,Kunkel TA,Scharff MD,Edelmann W||Proceedings of the National Academy of Sciences of the United States of America (110:E2470)||2013|
Zinc finger protein 668 interacts with Tip60 to promote H2AX acetylation after DNA damage.
MA1-26418 was used in immunocytochemistry and western blot to examine the interaction between zinc finger protein 668 interacts and and its effect on H2AX acetylation after DNA damage
|Hu R,Wang E,Peng G,Dai H,Lin SY||Cell cycle (Georgetown, Tex.) (12:2033)||2013|
Replication stress links structural and numerical cancer chromosomal instability.
MA1-26418 was used in immunocytochemistry to study the role of replication stress in cancer chromosomal instability
|Burrell RA,McClelland SE,Endesfelder D,Groth P,Weller MC,Shaikh N,Domingo E,Kanu N,Dewhurst SM,Gronroos E,Chew SK,Rowan AJ,Schenk A,Sheffer M,Howell M,Kschischo M,Behrens A,Helleday T,Bartek J,Tomlinson IP,Swanton C||Nature (494:492)||2013|
REV1 and polymerase ¿ facilitate homologous recombination repair.
MA1-26418 was used in immunocytochemistry to study the mechanisms by which REV1 and polymerase-zeta contribute to homologous recombination repair
|Sharma S,Hicks JK,Chute CL,Brennan JR,Ahn JY,Glover TW,Canman CE||Nucleic acids research (40:682)||2012|
Deficiency of FANCD2-associated nuclease KIAA1018/FAN1 sensitizes cells to interstrand crosslinking agents.
MA1-26418 was used in immunocytochemistry to study the role of KIAA1018/FAN1 depletion in increased cell sensitivity to DNA interstrand cross-linking agents
|Kratz K,Schöpf B,Kaden S,Sendoel A,Eberhard R,Lademann C,Cannavó E,Sartori AA,Hengartner MO,Jiricny J||Cell (142:77)||2010|
The cohesin complex is required for the DNA damage-induced G2/M checkpoint in mammalian cells.
MA1-26418 was used in immunocytochemistry to study the role of the cohesin complex in the DNA damage-induced G2/M checkpoint in mammalian cells
|Watrin E,Peters JM||The EMBO journal (28:2625)||2009|
Differential contribution of HP1 proteins to DNA end resection and homology-directed repair.
MA1-26418 was used in western blot to study the roles of human HP1 paralogues in homology-directed repair
|Soria G,Almouzni G||Cell cycle (Georgetown, Tex.) (12:422)||2013|
ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968.
MA1-26418 was used in western blot to investigate the effect of ST1968 on the induction of DNA damage response
|Zuco V,Benedetti V,Zunino F||Cancer letters (292:186)||2010|
Sensitization of ovarian carcinoma cells to the atypical retinoid ST1926 by the histone deacetylase inhibitor, RC307: enhanced DNA damage response.
MA1-26418 was used in western blot to investigate the therapeutic benefit of histone deacetylase inhibitor RC307 in the treatment of ovarian carcinoma with ST1926
|Zuco V,Benedetti V,De Cesare M,Zunino F||International journal of cancer (126:1246)||2010|
Ionizing radiation-dependent and independent phosphorylation of the 32-kDa subunit of replication protein A during mitosis.
MA1-26418 was used in western blot to study ionizing radiation-dependent phosphorylation of the 32-kDa subunit of replication protein A during mitosis
|Stephan H,Concannon C,Kremmer E,Carty MP,Nasheuer HP||Nucleic acids research (37:6028)||2009|
Gamma-glutamyltransferase-dependent resistance to arsenic trioxide in melanoma cells and cellular sensitization by ascorbic acid.
MA1-26418 was used in western blot to investigate the effect of some combination of prooxidant agents on overcoming melanoma cellular tolerance to oxidative stress
|Giommarelli C,Corti A,Supino R,Favini E,Paolicchi A,Pompella A,Zunino F||Free radical biology and medicine (46:1516)||2009|
Cellular response to oxidative stress and ascorbic acid in melanoma cells overexpressing gamma-glutamyltransferase.
MA1-26418 was used in western blot to investigate the role of gamma-glutamyltransferase in drug resistence toward cancer chemotherapy agents
|Giommarelli C,Corti A,Supino R,Favini E,Paolicchi A,Pompella A,Zunino F||European journal of cancer (Oxford, England : 1990) (44:750)||2008|
Mobility and distribution of replication protein A in living cells using fluorescence correlation spectroscopy.
MA1-26418 was used in western blot to investigate the mechanistic processes of replication protein A fucntion in DNA metabolism
|Braet C,Stephan H,Dobbie IM,Togashi DM,Ryder AG,Földes-Papp Z,Lowndes N,Nasheuer HP||Experimental and molecular pathology (82:156)||2007|
Development of resistance to the atypical retinoid, ST1926, in the lung carcinoma cell line H460 is associated with reduced formation of DNA strand breaks and a defective DNA damage response.
MA1-26418 was used in western blot to investigate the mechanism for the development of ST1926 resistence in lung carcinoma cell line H460
|Zuco V,Zanchi C,Lanzi C,Beretta GL,Supino R,Pisano C,Barbarino M,Zanier R,Bucci F,Aulicino C,Carminati P,Zunino F||Neoplasia (New York, N.Y.) (7:667)||2005|
The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene.
MA1-26418 was used in western blot to study the interaction between a replication protein A subunit and MEN1 tumor suppressor gene product
|Sukhodolets KE,Hickman AB,Agarwal SK,Sukhodolets MV,Obungu VH,Novotny EA,Crabtree JS,Chandrasekharappa SC,Collins FS,Spiegel AM,Burns AL,Marx SJ||Molecular and cellular biology (23:493)||2003|
Cyclin-dependent kinase suppression by WEE1 kinase protects the genome through control of replication initiation and nucleotide consumption.
MA1-26418 was used in flow cytometry to study the role of CDK in the mechanism by which WEE1 kinase maintains genomic integrity
|Beck H,Nähse-Kumpf V,Larsen MS,O'Hanlon KA,Patzke S,Holmberg C,Mejlvang J,Groth A,Nielsen O,Syljuåsen RG,Sørensen CS||Molecular and cellular biology (32:4226)||2012|
Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities.
MA1-26418 was used in immunohistochemistry and western blot to study the mechanisms by which human Fbh1 helicase contributes to genome maintenance
|Fugger K,Mistrik M,Danielsen JR,Dinant C,Falck J,Bartek J,Lukas J,Mailand N||The Journal of cell biology (186:655)||2009|
The NER proteins are differentially expressed in ever smokers and in never smokers with lung adenocarcinoma.
MA1-26418 was used in immunohistochemistry to investigate ERCC1, RPA and XPF expression in different types of smokers with lung adenocarcinoma
|Planchard D,Domont J,Taranchon E,Monnet I,Tredaniel J,Caliandro R,Validire P,Besse B,Soria JC,Fouret P||Annals of oncology : official journal of the European Society for Medical Oncology (20:1257)||2009|
Distinct functions of POT1 at telomeres.
MA1-26418 was used in immunohistochemistry to investigate the protective role of POT1 in telomeres
|Barrientos KS,Kendellen MF,Freibaum BD,Armbruster BN,Etheridge KT,Counter CM||Molecular and cellular biology (28:5251)||2008|
Human HMGB1 directly facilitates interactions between nucleotide excision repair proteins on triplex-directed psoralen interstrand crosslinks.
MA1-26418 was used in EMSA to investigate the role of HMGB1 in Psoralen-induced DNA damage repair
|Lange SS,Reddy MC,Vasquez KM||DNA repair (8:865)||2009|
Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha.
MA1-26418 was used in immunoprecipitation to study the role of the DNA replication factor Mcm10 in regulating DNA polymerase alpha stability and chromatin association
|Ricke RM,Bielinsky AK||Molecular cell (16:173)||2004|