|Tested species reactivity||Bovine, Dog, Chicken, Human, Mouse, Pig, Rat, Xenopus|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Bovine RPE65 microsomal membrane proteins.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:250-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250-1:500|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Highly conserved among vertebrate species, RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.
MA1-16578 was used in immunocytochemistry to examine the in vitro differentiation capacity of human bone marrow-derived stem cells to develop into retinal lineages.
|Mathivanan I,Trepp C,Brunold C,Baerlocher G,Enzmann V||Experimental cell research (333:11)||2015|