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Proteintech
RRP8 Monoclonal Antibody (1F5C2)
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FIGURE: 1 / 1
RRP8 Antibody (68382-1-IG) in WB
Various lysates were subjected to SDS PAGE followed by western blot with 68382-1-Ig (RRP8 antibody) at dilution of 1:10,000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
68382-1-IG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1F5C2
Immunogen
RRP8 fusion protein Ag16803
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
150 µg
Purification
Protein G
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: QKQGPPCSDS EEEVERKKKC HKQALVGSDS AEDEKRKRKC QKHAPINSAQ HLDNVDQTGP KAWKGSTTND PPKQSPGSTS PKPPHTLSRK QWRNRQKNKR RCKNKFQPPQ VPDQAPAEAP TEKTEVSPVP RTDSHEARAG ALRARMAQRL DGARFRYLNE QLYSGPSSAA QRLFQEDPEA FLLYHRGFQS QVKKWPLQPV DRIARDLRQR PASLVVADFG CGDCRLASSI RNPVHCFDLA SLDPRVTVCD MAQVPLEDES VDVAVFCLSL MGTNIRDFLE EANRVLKPGG LLKVAEVSSR FEDVRTFLRA VTKLGFKIVS KDLTNSHFFL FDFQKTGPPL VGPKAQLSGL QLQPCLYKRR
Target Information
Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cerebral protein 1; cerebral protein 1 homolog; HUCE1; hucep1; KIAA0409; nucleomethylin; nucleomethylin protein; ribosomal RNA processing 8, methyltransferase, homolog; RRP-8; RRP8 methyltransferase homolog; unnamed protein product
Gene Aliases: 1500003O22Rik; 2900001K19Rik; KIAA0409; NML; RGD1308302
UniProt ID: (Human) O43159, (Rat) Q5U4F0, (Mouse) Q9DB85
Entrez Gene ID: (Human) 23378, (Rat) 308911, (Mouse) 101867
chromatin silencing at rDNA
cellular response to glucose starvation
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
regulation of transcription by glucose
intrinsic apoptotic signaling pathway by p53 class mediator
energy homeostasis
regulation of G1 to G0 transition
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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