Immunofluorescent analysis of RSK1 p90 showing staining in the cytoplasm and nucleus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RSK1 p90 polyclonal antibody (Product # PA5-21699) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contain a sequence corresponding to a region within amino acids 303 and 527 (Q15418) of RSK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-21699 targets RSK1 p90 in IF and WB applications and shows reactivity with Human samples.
The PA5-21699 immunogen is recombinant protein fragment contain a sequence corresponding to a region within amino acids 303 and 527 (Q15418) of RSK1.
IP-MS enrichment of RPS6KA1 (LFQ intensity): RSK1 was enriched 619-fold from MCF7 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and RSK1 antibody (Part No. PA5-21699). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
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