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RTN1 (Isoform RTN1-C) Antibody (15048-1-AP) in IHC (P)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using (Product # 15048-1-AP) (RTN1 (Isoform RTN1-C) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Product Details
15048-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RTN1 (Isoform RTN1-C) Fusion Protein Ag7031 (1-208 aa encoded by BC000314)
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is raised against the full-length protein of human RTN1-C.
Immunogen sequence: MQATADSTK MDCVWSNWKS QAIDLLYWRD IKQTGIVFGS FLLLLFSLTQ FSVVSVVAYL ALAALSATIS FRIYKSVLQA VQKTDEGHPF KAYLELEITL SQEQIQKYTD CLQFYVNSTL KELRRLFLVQ DLVDSLKFAV LMWLLTYVGA LFNGLTLLLM AVVSMFTLPV VYVKHQAQID QYLGLVRTHI NAVVAKIQAK IPGAKRHAE (1-208 aa encoded by BC000314 )
Target Information
Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i. e. reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight of ~135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is so far not found in human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Neuroendocrine-specific protein; Reticulon-1; S-rex
Gene Aliases: NSP; RTN1
UniProt ID: (Human) Q16799, (Rat) Q64548, (Mouse) Q8K0T0
Entrez Gene ID: (Human) 6252, (Rat) 116644, (Mouse) 104001
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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