|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 445-474 amino acids surrounding S465 of human RUNX2|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
Runx2 is a member of the RUNX family of transcription factors. It is a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in the Runx2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Thyroid Hormone Receptor-ß (TRß) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.
PA5-14816 was used in ChIP assay to characterize a novel signaling pathway in thyroid cancer that mediates runt-related transcription factor 2 (Runx2) expression in thyroid cancer cells by thyroid hormone receptor beta (TRbeta)
|Carr FE,Tai PW,Barnum MS,Gillis NE,Evans KG,Taber TH,White JH,Tomczak JA,Jaworski DM,Zaidi SK,Lian JB,Stein JL,Stein GS||Endocrinology (157:3278)||2016|