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FIGURE: 1 / 1
RWDD3 Antibody (TA811763) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RWDD3 (RC217602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-RWDD3. Positive lysates LY414519 (100 µg) and LC414519 (20 µg) can be purchased separately from OriGene.
Product Details
TA811763
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
OTI10H4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-267 of human RWDD3 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases Ikappa-Beta sumoylation and stability. In addition, RWDD3 inhibits TNF-a-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of Ikappa-Beta increased stability. RSUME-enhanced sumoylation of Ikappa-Beta leads to the inhibition of NF-kappa-Beta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RSUME; RWD domain-containing protein 3; RWD domain-containing sumoylation enhancer; RWD-containing sumoylation enhancer
Gene Aliases: RSUME; RWDD3
UniProt ID: (Human) Q9Y3V2
Entrez Gene ID: (Human) 25950
Performance Guarantee
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