Immunofluorescence analysis of Rab11 was done on 80% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with ABfinity™ Rab11 Recombinant Rabbit Monoclonal Antibody (700184) at 2 µg-4 µg in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour® 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic localization of Rab11. Panel e shows no primary antibody. The images were captured at 20X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 179-194 of P62491.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2-4µg/10^6 cells|
|Western Blot (WB)||1-3µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
This antibody is predicted to react with bovine, chicken, equine, mouse, primate, rat, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Rab11A is a member of the YPT-1 subfamily of Ras-related GTP-binding proteins. Rab11A mRNA has been detected in a variety of tissues including brain, testis, spleen, heart, and gastrointestinal mucosa. In the gastric fundus the Rab11A protein is expressed in parietal cell tubulovesicles membranes. Expression of the Rab11A protein appears to occur in discrete epithelial cell populations where it localizes to apical vesicular populations. Recent studies have shown Rab11A is essential for resecretion of alpha-synuclein out of neuronal cells via endosome recycling and is required for exocytosis of discoidal/fusiform vesicles of bladder umbrella cells. Rab11A is also implicated in biogenesis of Birbeck granules and endosomal activation of the PI3K/AKT signaling pathway via G beta gamma trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CCC- and WASH-mediated endosomal sorting of LDLR is required for normal clearance of circulating LDL.
700184 was used in western blot to analyze how WASH- and CCC-mediated endosomal sorting of LDLr is needed for regular clearance of circulating LDL
|Bartuzi P,Billadeau DD,Favier R,Rong S,Dekker D,Fedoseienko A,Fieten H,Wijers M,Levels JH,Huijkman N,Kloosterhuis N,van der Molen H,Brufau G,Groen AK,Elliott AM,Kuivenhoven JA,Plecko B,Grangl G,McGaughran J,Horton JD,Burstein E,Hofker MH,van de Sluis B||Nature communications (7:null)||2016|
Exogenous ¿-synuclein decreases raft partitioning of Cav2.2 channels inducing dopamine release.
700184 was used in western blot to test if alpha-synuclein modulates neurotransmitter release
|Ronzitti G,Bucci G,Emanuele M,Leo D,Sotnikova TD,Mus LV,Soubrane CH,Dallas ML,Thalhammer A,Cingolani LA,Mochida S,Gainetdinov RR,Stephens GJ,Chieregatti E||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:10603)||2014|