Western blot analysis was performed on whole cell extracts (30 ug lysate) of HeLa (Lane 1) and PC-3 (Lane 2). The blots were probed with ABfinity TM Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product# 702211, 2 ug/ml) and detected by chemiluminescence using Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # 31458) at dilutions 1:5,000 (Fig. 1), 1:10,000 (Fig. 2) and 1:50,000 (Fig. 3). A 25 kDa band corresponding to PRDX6 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Storage Conditions||4° C|
|Cross Adsorption||Against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000-1:200,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, and ICC applications.
Antibody Specificity: This antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat and sheep serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 2-8°C until opened. Restore with 0.5 ml distilled water (0.8 mg/ml after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at -70°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Thermo Scientific Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tracking the dispersion of Scaphoideus titanus Ball (Hemiptera: Cicadellidae) from wild to cultivated grapevine: use of a novel mark-capture technique.
31458 was used in ELISA to use a novel tracking technique to monitor the movement of Scaphoides titanus Ball from wild to cultivated grapevines
|Lessio F,Tota F,Alma A||Bulletin of entomological research (104:432)||2014|
|Not Applicable||Not Cited||
Use of a highly sensitive immunomarking system to characterize face fly (Diptera: Muscidae) dispersal from cow pats.
31458 was used in ELISA to study the dispersal of face flies from cow pats using an immuno-based marking and detection system
|Peck GW,Ferguson HJ,Jones VP,O'Neal SD,Walsh DB||Environmental entomology (43:116)||2014|
Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration.
31458 was used in western blot to study the increased levels of certain axon guidance proteins in the CNS of Nogo-A ablated mice and their role in limiting the outgrowth of neurites in the absence of Nogo
|Kempf A,Montani L,Petrinovic MM,Schroeter A,Weinmann O,Patrignani A,Schwab ME||The European journal of neuroscience (38:3567)||2013|