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Immunofluorescence analysis of PRDM14 (green) in a human iPSC line, Gibco Episomal iPSC line (Product # A18945) grown on vitronectin in Essential 8™ medium (Product # A1517001). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a PRDM14 polyclonal antibody (Product # PA1-114) at a dilution of 1:500 in 3% BSA/PBS blocking buffer overnight at 4°C, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35553) at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). Images were taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification.
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against human, horse, mouse, swine, goat and rat serum proteins|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25 - 1:100|
|Immunocytochemistry (ICC)||1:50 - 1:2,000|
|Immunofluorescence (IF)||1:50 - 1:2,000|
|Immunohistochemistry (IHC)||1:50 - 1:2,000|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:5,000 - 1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Product # 35553 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 35553 reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by solid-phase adsorbed to ensure minimal cross-reactivity with human, horse, mouse, swine, goat, and rat serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product protected from light at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C. DyLight 488 Amax= 493 nm; Emax= 518 nm. Mole Dye/Mole Protein Ratio is lot-dependent.
Thermo Scientific Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tackling obstacles for gene therapy targeting neurons: disrupting perineural nets with hyaluronidase improves transduction.
35553 was used in immunocytochemistry to study the potential utility for gene therapy of co-administering hyaluronidase and lentiviral vectors to improve the transduction efficiency of neuronal cells
|Wanisch K,Kovac S,Schorge S||PloS one (8:null)||2013|