Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1) and PC-3 (Lane 2). The blots were probed with ABfinity TM Anti-PRDX6 Recombinant Rabbit Monoclonal Antibody (Product # 702211, 2 µg/ml) and detected using Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP conjugate (Product # SA1-200) at dilutions 1:60 (Fig. 1), 1:400 (Fig. 2) and 1:1,000 (Fig. 3). A 25 kDa band corresponding to PRDX6 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Storage buffer||PBS with 50% glycerol, proprietary stabilizer|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Cross Adsorption||Against bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat and sheep serum proteins|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:60-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
Product SA1-200 is conjugated to HRP and optimized for ECL applications. This antibody reacts with the heavy and light chains of the immunoglobulin molecule. Pre-absorbed with the following species proteins: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat, and sheep serum proteins.
Thermo Scientific secondary antibodies are conjugated to Horseradish-Peroxidase (HRP) for use in Western blot applications. These antibodies are affinity-purified and optimized for use in ECL experiments and have been absorbed against the serum proteins of a large number of species to virtually eliminate background and non-specific staining.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Lipidomic analyses of female mice lacking hepatic lipase and endothelial lipase indicate selective modulation of plasma lipid species.
SA1-200 was used in western blot to study the lipidomics of mice deleted for hepatic lipase and/or endothelial lipase to determine the contribution of each lipase to the hydrolysis of individual lipid species
|Yang Y,Kuwano T,Lagor WR,Albert CJ,Brenton S,Rader DJ,Ford DA,Brown RJ||Lipids (49:505)||2014|
|Not Applicable||Not Cited||
The influence of CpG and UpA dinucleotide frequencies on RNA virus replication and characterization of the innate cellular pathways underlying virus attenuation and enhanced replication.
SA1-200 was used in western blot to study the replication of RNA viruses and the significance of the frequency of CpG and UpA dinucleotides
|Atkinson NJ,Witteveldt J,Evans DJ,Simmonds P||Nucleic acids research (42:4527)||2014|
Hydrolysis products generated by lipoprotein lipase and endothelial lipase differentially impact THP-1 macrophage cell signalling pathways.
SA1-200 was used in western blot to study the differential effects on macrophage signaling of lipoprotein hydrolysis products produced by lipoprotein lipase and endothelial lipase respectively
|Essaji Y,Yang Y,Albert CJ,Ford DA,Brown RJ||Lipids (48:769)||2013|
Dynamic changes in expression of myosin phosphatase in a model of portal hypertension.
SA1-200 was used in western blot to compare MYPT1 isoforms' expression level and smooth muscle's corresponding phenotype in normal rat vessels with those in a prehepatic model of portal hypertension
|Payne MC,Zhang HY,Shirasawa Y,Koga Y,Ikebe M,Benoit JN,Fisher SA||American journal of physiology. Heart and circulatory physiology (286:H1801)||2004|