Western blot analysis of LAMP1 was performed by loading 20 µg of HeLa cell lysate and 5 µl of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-LAMP1 polyclonal antibody (Product # PA1654A) at a dilution of 1:500 for one hour at room temperature, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 32260) at a dilution of 1:100,000 for 1hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with proprietary stabilizer|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:60-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody may cross-react with immunoglobulins from other species.
Thermo Scientific Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Glutamine Improves Innate Immunity and Prevents Bacterial Enteroinvasion During Parenteral Nutrition.
32260 was used in western blot to study the mechanisms by which glutamine supplementation of parenteral nutrition boosts innate immunity and protects against bacterial mucosal invasion
|Wang X,Pierre JF,Heneghan AF,Busch RA,Kudsk KA||JPEN. Journal of parenteral and enteral nutrition (39:688)||2015|