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Endogenous Histone H3[AcK9] (red) was labeled with Rabbit anti-Histone H3[AcK9] primary antibody (710293) (1 ug/ml) (top panel) to assess signal or no primary antibody to assess background (bottom panel) in U20S human osteocarcinoma cells and visualized using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 555 conjugate (A27039) (0.4 ug/ml, 1:2500). To demonstrate multiplexing Tubulin (green) was labeled with anti-Tubulin (322500) mouse primary antibody and visualized with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) (1 ug/ml).
|Tested species reactivity||Rabbit|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant full-length protein|
|Conjugate||Alexa Fluor® 555|
|Purification||Gravity column chromatography|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||0.1-2 µg/ml|
|Immunofluorescence (IF)||0.1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig and donkey IgG is observed.
Superclonal™ Secondary Antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced sensitivity and specificity to the target species IgG. These recombinant secondary antibodies show exceptional performance in common immunoassay applications, with very little cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin ensures lot-to-lot consistency, hence eliminating the need to optimize each lot in relevant assays. Our Superclonal™ secondary antibodies allow scientists to achieve the accurate results they need, without the cross-reactivity concerns that can impact some traditional polyclonal secondary antibodies. Superclonal™ secondaries are available with Alexa Fluor conjugates, as well as HRP, AP and biotin. The Alexa Fluor dyes have set the industry standard for brightness and photostability for several years. Alexa Fluor secondary antibodies are trusted, having been used in over 30,000 peer reviewed papers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.